Phellsgate 2

Author: nsfq

August undefined, 2024

Web2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g. 5.Store the plasmid at -20 ℃. pHELLSGATE 8 vector Sequence Copy Sequence WebIn this paper we describe two improved pHellsgate vectors that facilitate rapid generation of hpRNA-encoding constructs. pHellsgate 4 allows the production of an hpRNA construct in a single step from a single polymerase chain reaction product, while pHellsgate 8 requires a two-step process via an intermediate vector.

Does anyone having the intermediate pHANNIBAL,pKANNIBAL

Web(Invitrogen), and 150 ng plasmid of pHellsgate 2. The reaction was held at room temperature (25 C) for 4 h. Thereafter, 1 lL proteinase K was added and incubated at 37 C for 10 min to stop the reaction. About 2 lL mix was used to transform the Escherichia coli DH10B. The plas-mids of positive clones on the plate with spectinomycin should i help judy take over clouds

Constructs and methods for high-throughput gene silencing in …

WebAug 1, 2003 · The pHELLSGATE vectors A high-throughout cloning system to generate hpRNA constructs requires a vector, such as pHANNIBAL, to give directional insertion of a gene fragment into an inverted repeat conformation separated by an intron. WebDec 23, 2001 · The pHELLSGATE vector was tested using 200 and 400 nt PCR products for two different genes. Many bacterial colonies were obtained on chloramphenicol … WebpHELLSGATE, pWATERGATE, pSTARGATE and pOpOff contain Gateway TM recombination sites. Gateway TM is proprietary technology owned by Invitrogen and to the extent that CSIRO vectors incorporate Gateway TM technology, ... 5.2 ALL TERMS, CONDITIONS AND WARRANTIES (INCLUDING ANY WARRANTY AS TO MERCHANTABLE QUALITY OR … should i help kraityn

Analysis of the hairpin RNA targeting PnPR-like ... - ResearchGate

Frontiers A High-Throughput Standard PCR-Based Genotyping …

WebAfter inoculation with F. solani for 72 h, the PnPR-like expression level in the control leaves was about 5-times higher than that in the leaves transformed with pHellsgate 2-PnPR-like, which ... WebWe have previously mapped Wo to an approximately 200-kb fragment by using an F 2 population from a cross between an introgression line 2–3 and woolly mutant LA3186 ... Suppression expressions of Wo and SlCycB2 were performed by using RNAi vector pHellsgate 2, which were ligated with the fragment of Wo and SlCycB2 (amplified with the … satisfactory stuck in terrainWebpHELLSGATE 12 High-throughput binary Gateway® vector with two introns, for silencing genes in plants using intron-containing hairpin RNA. Open in SnapGene Try SnapGene for … satisfactory spielstand finden

"WebMar 20, 2009 · The amplicons were cloned in pHELLSGATE 2 using BP and LR clonase. This protocol allows the insertion, in one step, of the genes of interest in sense and antisense orientation with an intron in... " - Phellsgate 2

Phellsgate 2

Constructs and methods for high-throughput gene silencing in …

WebpHELLSGATE 12 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … WebThe system utilizes a modified pHELLSGATE vector, under the control of the pOp6 promoter, and the synthetic transcription factor, LhGR. We demonstrate that the production of RNAi …

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WebSep 9, 2024 · PAL1 gene was amplified from cassava cDNA using primers with 5′ adaptor (attB) sequences and cloned into pHELLSGATE vector (CISRO, Australia) using Gateway cloning to prepare the RNAi construct (Additional file 1: Table S1) . pHELLSGATE-PAL1 RNAi construct was transformed into competent cells of the super virulent Agrobacterium … WebAlternatively, generic gene-silencing vectors such as the pHANNIBAL and the pHELLSGATE series can be used. They simply require the insertion of PCR products, derived from the target gene, into the vectors by conventional cloning or by using the Gateway directed recombination system.

WebpHELLSGATE is an efficient, high-throughput and effective plant gene silencing vector. Vector Type. Gene Silencing Vector, Gateway Vector, Plant Dual Expression Vector ... Powder; Solution Size. 2 μg; 10 μL . High Copy/Low Copy. High Copy . Prokaryotic Resistance. Spectinomycin . Length. 18691 bp . Selectable Marker. WebpHELLSGATE载体价格10000元，pHELLSGATE载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性壮观霉素或链霉素，质粒大小为18691BP，测序引物见 …

WebThe pHELLSGATE vectors contain two recombination cassettes consisting of either attP1-ccdB-attP2 or attR1-ccdB-attR2 in an inverted repeat configuration, such that when gene fragments flanked by the appropriate att sites are recombined with the vector, an ihpRNA-encoding construct is produced ( Fig. 3 ). WebAug 1, 2003 · The pHELLSGATE vectors contain convenient restriction sites that can be used to check for the presence of inserted sequences and diagnose the orientation of the …

WebSep 19, 2016 · a, Total RNA extracted from the N. benthamiana plants expressing Hellsgate empty vector (EV), YFP or Bc-DCL1/2 RNAi constructs was examined by northern blot analysis to measure the expression...

WebSep 28, 2010 · pHELLSGATE is an RNAi vector used to validate gene function in plants. In the present study, recombinant pHELLSGATE vector was constructed with amplicons of the gene serine threonine protein kinase (STPK) cloned from Piper colubrinum. should i hedge my etfWebBioz Stars score, Techniques, Protocol Conditions and more for Binary Vector Phellsgate 2, supplied by Thermo Fisher. Data for Binary Vector Phellsgate 2 gathered from related … satisfactory stuck on cloud syncingWebThe pHELLSGATE vectors contain two recombination cassettes consisting of either attP1-ccdB-attP2 or attR1-ccdB-attR2 in an inverted repeat configuration, such that when gene … satisfactory steam api failed to initializeWebJul 24, 2024 · The optimization of the pHellsgate8-specific primer pairs 35S_S, OCS_S, and NPTII-3 centered on manipulating the following parameters: (i) final concentration of MgCl 2 in the PCR reaction, (ii) number of amplification cycles in the PCR program, and (iii) single versus multiplex PCR. satisfactory tier 8Web4 beds, 3 baths, 2278 sq. ft. house located at 2302 Fieldgate Dr, Pittsburg, CA 94565. View sales history, tax history, home value estimates, and overhead views. APN ... should i help gareth weasleyWebJan 2, 2015 · Cinnamyl AlcoholDehydrogenase (CAD) is the final enzyme involved in the monolignol biosynthesispathway.Transgenic poplars were produced using RNAi strategy (pHellsgate 8 vector, Helliwell and Waterhouse, 2003) and exhibited the expectedphenotype (red xylem, reduced CAD activity). -RNAi-CAD transgenic poplars were produced using … should i help my boyfriend financiallyWebSep 19, 2016 · The plasmids pHellsgate-Bc-DCL1/2 and pHellsgate-Bc+Vd-DCLs were constructed following the methods outlined for the Hellsgate8.0 system 51. The Bc-DCL1/2 RNAi fragment was obtained by integrating Bc-DCL1 (252 bp) and Bc-DCL2 (238 bp) via overlapping PCR. satisfactory speed up hyper tube